Cynosurus indicus L., Eleusine distans Moench, E. gracilis Salisb.) The MeTH was showed to have the highest TPC and scavenging activity (77.7%) on DPPH assay, followed by EA (64.5%), hexane (47.19%) and DCM (40.83%) extracts, whereas the MeTH showed no inhibitory effect on all tested bacteria strains. Mierziak J, Koslyn K, Kulma A. Flavonoids as Important Molecules of Plant Interactions with the Environment.
Eleusine indica L. (Gaertn), commonly known as goosegrass, is a monocot weed belonging to the Poaceae family . the EI extract concentration reducing the absorbance of treated cells by 50% in respect to untreated cells). It is commonly called kodo in Nepal where 877 accessions have been maintained by National Plant Genetic Resource Centre, Khumaltar, Nepal. Finally, the combined extracts for each solvent were filtered through Whatman No. Seeds of Eleusine indica buried at soil depths of 0, 0.5, 1, 2, 4 and 6 cm germinated to 82, 45, 32, 22, 12 and 0%, respectively (Chauhan and Johnson, 2008f). https://doi However, the EA extract exhibited a broad spectrum antibacterial activity against all tested bacteria except Bacillus subtilis, in which this bacterium was found to be resistant to all EI extracts. Sterile discs were impregnated with the extract solutions (0.05?ml from 100?mg/ml extract) and placed in inoculated agar. It has been reported previously that some plant extracts have shown to have multi-biological activities of possessing both antibacterial and antioxidant activities [35–37]. Due to its high fecundity and a wide tolerance to various environmental factors, goosegrass is listed as one of Our results revealed that there is a strong and significant correlation between TPC and DPPH free radical scavenging activity of the EI extracts. Learn about our remote access options, Department of Crop, Soil and Environmental Science, Auburn University, Auburn, AL, USA, Department of Biological Sciences, Auburn University, Auburn, AL, USA, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, USA, Georgia Institute of Technology, School of Biological Sciences, Atlanta, GA, USA, Correspondence to: JS McElroy, Department of Crop, Soil and Environmental Science, Auburn University, Alabama, USA. 2623-2630 ISSN: 1520-5118 The working solution was prepared in methanol at concentration of 500?g/ml. Figure S3. Seed germination in goose grass (Eleusine indica). Kim, P. Seguin, J.-K. Ahn et al., “Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea,”, P. Middleton, F. Stewart, S. Al-Qahtani et al., “Antioxidant, antibacterial activities and general toxicity of, S. F. Van Vuuren, “Antimicrobial activity of South African medicinal plants,”, J. Mc Farland, “Standardization of bacterial culture for disc diffusion assay,”, I. Gülçin, M. Oktay, E. Kireçci, and Ö. I. Küfrevioǧlu, “Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts,”, A. Turkoglu, M. E. Duru, N. Mercan, I. Kivrak, and K. Gezer, “Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Any queries (other than missing content) should be directed to the corresponding author for the article. 1, 2018 90 ISSN 2704-3568 Antiurolithiatic activity of ethanolic extract of Eleusine indica Linn. Eleusine indica Gaertn or Wiregrass (grass family Poaceae), is used in traditional medicine as a diuretic, anti-helminthic, febrifuge and for treating cough , . 0%, 1%, 2% and 3% potted treatments of Copper and Nickel contaminated soil were made for three samples (initial … The BUSCO results: 95.0% complete single‐copy orthologs, 1.9% complete duplicated, 1.2% fragmented and 1.9% missing of orthologs in goosegrass genome.
Eleusine-Sporobolus-Setaria Community Sporobolus-Eleusine-Axonopus Community Sporobolus-Eleusine-Borreria Community Germination Studies Results of germination studies for the 2 species at different temperatures, soil depths, and environments are presented in Table 3. The stem or the leaf sheath (part where E. indica seeds germinated between 20°C and WC with opti- Cooperative Extension, which staffs local offices in all 100 counties and with the Eastern Band of Cherokee Indians. Finally, the absorbance was read at wavelength of 595?nm using a microtitre plate reader (Labsystems iEMS Reader MF) and the percentage cell viability was calculated with the appropriate controls taken into account. Journal of Biodiversity and Environmental Sciences 13(4), 131-140. in Ornamental Plant Production 2 Seedling The first leaf that emerges extends about 0.4 in (1 cm) in length and lies parallel to the soil. Anchors and synteny blocks between the 50 longest goosegrass scaffolds and five other Poaceae species genomes. ?ml of Folin-Ciocalteu reagent (1? Eleusine indica NC State University and N.C. A&T State University work in tandem, along with federal, state and local governments, to form a strategic partnership called N.C. Figure S1. Available online at www.ijpab.com ISSN: 2320 – 7051 Int. rumput belulang berkembang biak dengan menggunakan biji. manienie ali'I. It is invasive and highly adaptable to diverse habitats and crops. This species is accepted, and its native range is Tropical & Subtropical Old World. Antibacterial and teratogenic activity of Eleusine indica leaf extracts. The assembled genome contains 25,467 unique protein‐coding genes. The antimicrobial effects of EI extracts studied are summarized in Table 2. However, R biotypes of goosegrass have arisen due … This reaction involves DPPH reacting with an antioxidant compound, the latter donating hydrogen and reduced, which then leads to the change of DPPH colour from deep-violet to light-yellow. Then, the media was removed and DMSO was added into each well to solubilize the formazan crystals. To screen the extracts of EI, dried extracts of the plant were dissolved in 1?ml of DMSO to give a stock solution of extract at 10?mg/ml. Molecular approach as a herbicide-resistant agent detector is an initial solution before controlling of Eleusine indica weed.This review article is aimed to obtain the basic data of similarity and phylogenetic values among herbicide-resistant E. indica biotypes. Authentication was done at the same laboratory where the voucher specimen (EI-L100158) was deposited. These metabolites include the flavonoids, phenols and phenolic glycosides, saponins, cyanogenic glycosides, unsaturated lactones and glucosinolates [5–8]. On the other hand, the EA extract showed a broad spectrum activity, compared with the positive control (Streptomycin) against all tested bacteria except for B. subtilis, which showed resistance to all extracts of EI. Most orthogroups only include two species, followed by seven species. 1) Diffusion method [21, 22]. Cytotoxicity assay results revealed that the hexane, DCM, EA and MeTH of EI were found to have IC50 values of over 30?g/ml against MCF-7, HT-29 and CEM-SS cancer cell lines after 72?h incubation. Therefore, this study was conducted to evaluate the antioxidant, antibacterial and anti-cancer activities of the hexane, dichloromethane (DCM), ethyl acetate (EA) and methanol extracts (MeTH) of EI using total phenolic content (TPC), DPPH, disc diffusion and MTT cytotoxicity assay methods of analysis. by RNA sequencing technology. Human T4-lymphoblastoid cell line, CEM-SS, used in this study were obtained from The NIH AIDS Research and Reference Reagent Program, USA. The cytoxicity profiles of the extracts were assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) microculture tetrazolium viability assay as described by Mosmann . crowsfoot grass. Eleusine indica, etc ; 3-phosphoshikimate 1-carboxyvinyltransferase; antibodies; gene overexpression; genes; glyphosate; glyphosate resistance; herbicide-resistant weeds; mutation; ... Journal of agricultural and food chemistry 2020 v.68 no.9 pp. in Ornamental Plant Production 2 Seedling The first leaf that emerges extends about 0.4 in (1 cm) in length and lies parallel to the soil. Omics Potential in Herbicide-Resistant Weed Management. Biology and Management of Goosegrass (Eleusine indica (L.) Gaertn.) Eleusine indica weed is having to grow very fast on full sunlight intensity and classified C4 plants . Table S2. It is an invasive species in some areas. Eleucine indica (EI) is traditionally used in ailments associated with liver and kidneys. The radical scavenging activity of extracts was determined following the method of Changwei et al. A dose-response curve for the percentages of viability cells (0–100%) was plotted against concentrations of 0.469–60?g/ml of the extracts. Hence, for selecting crude plant extracts with potential useful properties, in vitro screening methods have been used for further in-depth chemical elucidation and pharmacological investigations . E. indica has been considered not only an important weed but also a possible donor of the sub‐genome A of the three allotetraploid species in the genus Eleusine, E. africana (2n = 4x = 36), E. coracana (2n = 4x = 36), and (2n = 4 This information can be used in biotechnology research which contains explanations about herbicides-resistant, including early detection and management for herbicides-resistant E. indica biotype. Databases of E. indica have one book and reports, 1 MeSH, 3 NLM catalog, 303 scientific and abstracts or citations, 838 full-text journal articles, and 744 nucleotide DNA and RNA sequences. Copyright © 2011 Adel S. Al-Zubairi et al. Eleusine indica, and Polyscias fulva. 5, p. 919. Studies have shown that C-glycosylflavones from Eleusine indica have anti-inflammatory effects on lipopolysaccharide-induced lung airway inflammation in mice . The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. The positive control, Streptomycin, had shown zones of inhibition of 20 1.5, 20 1.3, 23 1.5 and 23 1.0?mm in MRSA, P. aeruginosa, S. choleraesuis and B. subtilis, respectively. Other studies showed that there were significant correlations between phenolic compounds and antioxidant properties of medicinal plants under investigations [30, 31]. RESULTS A draft assembly of an approximately 492 Mb whole‐genome sequence of goosegrass was obtained by de novo assembly of paired‐end and mate‐paired reads generated by Illumina sequencing of total genomic DNA. Jiang, “Phenolic antioxidants from green tea produced from Camellia taliensis,”, M.-Y. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. where OD is the optical density.
Figure S6. The solvents used were hexane, DCM, EA and methanol. Cyhalofop-butyl and glyphosate multiple herbicide resistance evolved in an Eleusine indica population collected in Chinese direct-seeding rice.
The antibacterial activities of the different extracts of EI were evaluated using both Gram-positive and Gram-negative bacteria. The peak indicates the expected coverage for the sequenced single copy regions of the genome. 14(5), 909-918. 220.127.116.11.1 Origin of Finger Millet. Briefly, stock solutions of EI extracts were prepared in a concentration of 20?mg/ml. Phenolic compounds possess diverse biological activities such as anti-inflammatory, anti-carcinogenic and anti-atherosclerotic activities. Figure S2. Table S1. Zhang, C.-R. Yang, K.-K. Chen, and H.-J. As mentioned by Giam (2016), approximately three-quarters of human-attributed The powdered leaves (300?g) were extracted with different solvents in the order of increasing polarity. Fifty microliters of this solution was transferred to test tubes (). 2016;29: 1875–1878. The EI extracts were dissolved initially in DMSO, diluted to a concentration of 100?mg/ml and filtered using 0.45?m millipore filters. 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